Industriestrasse 25 CH-9471 Buchs E-mail: Flukatec@eurnotes.sial.com Fax: ++41 81 755 2815 85793 Tris(4,7-diphenyl-1,10-phenanthroline)-ruthenium hexafluorophosphate Ru(dpp)3(PF6)2 Application Superior lipid soluble luminescent oxygen probe (see below). Product Description Net formula C72H48F12N6P2Ru MW 1388.19 Appearance orange solid Solubility soluble in ethanol, methanol, chloroform, toluene (moderately) Quantity 1 mg, 5 mg Absorption and fluorescence emission of Ru(dpp)3(PF6)2 in a polystyrene film 1 relativeabsorption / emission 0 400 450 500 550 600 650 700 wavelength [nm] abs. max 455 nm, luminescence max 613 nm Quenchability by Oxygen: The fluorescence of Ru(dpp)3(PF6)2 is strongly reduced by molecular oxygen due to dynamic quenching. Therefore, it is a viable oxygen probe based on either measurement of intensity or decay time. Quenching usually is expressed as the ratio of the decay times in the absence (0) and presence (), respectively, of a quencher and can be presented as a Stern-Volmer plot as shown below. The graph says that, e.g., at a partial pressure of 200 hPa the decay time (and intensity) of Ru(dpp)3(PF6)2 have dropped by a factor of 2 due to dynamic quenching. Industriestrasse 25 CH-9471 Buchs E-mail: Flukatec@eurnotes.sial.com Fax: ++41 81 755 2815 The linear graph gives the ideal situation. In practice (for example if the probe is dissolved in a polymer), deviations from linearity are observed as shown in the graph. . 0/ . 3,0 2,5 2,0 1,5 1,0 0 100 200 300 400 500 pO2[hPa] Protocol for labelling polystyrene beads with Ru(dpp)3(PF6)2: Polystyrene beads (5 mg) are suspended in 20 mL of an methanol/water mixture (1/1, v/v). Then, a solution of 1 mg of the Ru complex in 0.5 mL of toluene is added and the mixture sonicated for 5 min. The suspension is stirred over night and filtered (to remove sticky beads). After addition of 20 mL of water, the solvent is reduced to 2 mL using a rotary evaporator under reduced pressure. The suspension is then placed in a 2 mL Eppendorf cup, centrifuged three times (washing with water) and finally suspended in distilled water.